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KMID : 0903519750180010052
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1975 Volume.18 No. 1 p.52 ~ p.55
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The Microdetermination of Ascorbic acid in Blood by Gas - Liquid Chromatography
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Abstract
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A microdetermination of Ascorbic acid (AsA) in blood by Gas-Liquid Chromatography (GLC) was studied. AsA was applied on GLC after the conversion into trimethylsilyl derivative (TMS) and the GLC was available for the only reduced form of AsA.
A calibration curve is made by GLC of TMS-AsA as the internal standard of n-docosane. The minimum amount of AsA required for the determination was 0.5㎖ of 1 ㎎% pyridine solution.
Prior to the conversion of AsA in serum into TMS derivative, serum was lyophilized and then it was allowed to stand at room temperature with TMS reagents for 48 hr. On injection of the supernates of TMS derivative to GLC the peak corresponding to AsA was not detected. Its reason why the concentration of AsA in serum is 0.5㎎∼0.8㎎% in general, and it is less than minimum concentration of GLC.
In case cf L-AsA 1 ㎎ was added to 1㎖ of serum, which was followed by lyophilization, silylation and GLC. The recorvery of AsA added was 98 percentage.
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